Genotype-habitat associations did not extend beyond soil, however. Chi-square analysis revealed mtDNA lineage predominance in particular soil textures, indicating the potential of soil to function as a limiting environmental factor in the distribution of lineages within this species. In this study, 3 mtDNA lineages were analyzed for their association with ecological variables, and PCR-RFLP haplotype genetic diversities were analyzed for relationships with elevation, elevation-latitude index, and meadow size. Previous analyses showed that genetic differentiation is not distance driven, so habitat factors may account for genetic structuring and diversity within the species. Melanoplus alpinus, a montane/alpine grasshopper species found in the central and northern Rocky Mountains, has a disjunct distribution with highly divergent mtDNA lineages, structured among meadows and drainages within mountain ranges. Such understanding may help to elucidate complex microevolutionary processes. Understanding the relationships between intraspecific diversity and habitat has been greatly enhanced with the advent of molecular markers.
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